This kit supplies ready-to-plate irradiated HFF-1 human foreskin fibroblasts (HFF) (ATCC SCRC 1041.1) as the feeder cell layer and cell culture components including Pluripotent Stem Cell SFM XF, Stem Cell Dissociation Reagent (ATCC ACS-3010), ROCK Inhibitor Y27632 (ATCC ACS-3030), Stem Cell Freezing Media (ATCC ACS-3020), DMEM: F12 Medium (ATCC 30-2006) and D-PBS (ATCC 30-2200)